Protein Microarrays Based on Single Framework Recombinant Antibody Fragments (sinfabs) Catcher and Carrier – a Crucial Combination
نویسنده
چکیده
ACADEMIC DISSERTATION submitted to the Technical Faculty of Lund University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY IN ENGINEERING. The public defense will take place in Lundmarksalen, Astronomic Institute, Sölvegatan 27, Lund, Friday, the 16th of December 2005, 13:15 p.m.. The Opponent of the Faculty is Dr. Jörg Hoheisel, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany.
منابع مشابه
Identification of protein expression signatures associated with H. pylori infection and gastric adenocarcinoma using recombinant antibody microarrays
Running Title: Protein expression profiling of normal and malignant tissue Summary Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immuno...
متن کاملIdentification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays.
Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody...
متن کاملConstruction of recombinant Pichia pastoris expressing single-chain antibody fragment against extracellular domain of EpCAM
Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...
متن کاملCloning and Expression of Recombinant Camelid Single-Domain Antibody in Tobacco
Antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. A promising alternative to conventional antibodies are the heavy chain antibodies (VHH) of Camelidae having short length, high solubility and stability are preferred to other antibody derivatives. In this study, our goal was product...
متن کاملProtein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry.
With the human genome in a first sequence draft and several other genomes being finished this year, the existing information gap between genomics and proteomics is becoming increasingly evident. The analysis of the proteome is, however, much more complicated because the synthesis and structural requirements of functional proteins are different from the easily handled oligonucleotides, for which...
متن کامل